منابع مشابه
Germ line chimeras from female ES cells.
Murine embryonic stem cells (ES cells) are pluripotent cells that can contribute to all tissues of developing mice including the germ line when aggregated with 8-cell-stage embryos or injected into the blastocoel cavity of murine blastocysts. As well as for in vitro studies, they are used to introduce mutations into the murine genome, thereby producing new mutant mouse lines. All ES cell lines ...
متن کاملEfficient Generation of Germ Line Transmitting Chimeras from C57BL/6N ES Cells by Aggregation with Outbred Host Embryos
Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every gene of the mouse genome. To realize the utility of these resources, efficient and accessible methods of generat...
متن کاملProduction of zebrafish germ-line chimeras from embryo cell cultures.
Although the zebrafish possesses many characteristics that make it a valuable model for genetic studies of vertebrate development, one deficiency of this model system is the absence of methods for cell-mediated gene transfer and targeted gene inactivation. In mice, embryonic stem cell cultures are routinely used for gene transfer and provide the advantage of in vitro selection for rare events s...
متن کاملisolation and enrichment of mouse female germ line stem cells
objective: the existence of female germ-line stem cells (fgscs) has been the subject of a wide range of recent studies. successful isolation and culture of fgscs could facilitate studies on regenerative medicine and infertility treatments in the near future. our aim in the present study was evaluation of the most commonly used techniques in enrichment of fgscs and in establishment of the best p...
متن کاملIsolation and Enrichment of Mouse Female Germ Line Stem Cells
OBJECTIVE The existence of female germ-line stem cells (FGSCs) has been the subject of a wide range of recent studies. Successful isolation and culture of FGSCs could facilitate studies on regenerative medicine and infertility treatments in the near future. Our aim in the present study was evaluation of the most commonly used techniques in enrichment of FGSCs and in establishment of the best pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Experimental Cell Research
سال: 1997
ISSN: 0014-4827
DOI: 10.1006/excr.1996.3418